Publications

2021

40. C. Petchakup, P.E. Hutchinson, H.M. Tay, S.Y. Leong, K.H.H. Li, H.W. Hou, Label-free quantitative lymphocyte activation profiling using microfluidic impedance cytometry, Sensors and Actuators B: Chemical, In-Press, 2021.

39. L. Gong, C. Petchakup, P. Shi, P.L. Tan, L.P. Tan, C.Y. Tay, H.W. Hou, Direct and Label‐Free Cell Status Monitoring of Spheroids and Microcarriers Using Microfluidic Impedance Cytometry, Small, 2007500, 2021.

2020

38. C. Liu, M.H.Y. Teo, S.L.T. Pek, Xi. Wu, M.L. Leong, H.M. Tay, H.W. Hou, C. Ruedl, S.E. Moss, J. Greenwood, S. Tavintharan, W. Hong, X. Wang, A Multifunctional Role of Leucine-Rich-Alpha 2 Glycoprotein 1 in Cutaneous Wound Healing Under Normal and Diabetic Conditions, Diabetes, 69(10), 2020.

37. Z. Wu, H.K. Lim, S.J. Tan, A. Gautam, H.W. Hou, K.W. Ng, N.S. Tan, C.Y. Tay, Potent‐By‐Design: Amino Acids Mimicking Porous Nanotherapeutics with Intrinsic Anticancer Targeting Properties, Small, In-press, 2020.

36. N. Menon, C. Su, K.T. Pang, Z.J. Phua, H.M. Tay, R. Dalan, X. Wang, K.H.H. Li and H.W. Hou, Recapitulating Atherogenic Flow Disturbances and Vascular Inflammation in a Perfusable 3D Stenosis Model, Biofabrication, In-press, 2020.

35. H.M. Tay, W.H. Yeap, R. Dalan, S.C. Wong* and H.W. Hou*, Increased monocyte‐platelet aggregates and monocyte‐endothelial adhesion in healthy individuals with vitamin D deficiency, FASEB, 34(8): 11133-11142, 2020.

2019

34. N. Liu, C. Petchakup., H.M. Tay, K.H.H. Li, H.W. Hou (2019) Spiral Inertial Microfluidics for Cell Separation and Biomedical Applications. In: Tokeshi M. (eds) Applications of Microfluidic Systems in Biology and Medicine. Bioanalysis (Advanced Materials, Methods, and Devices), vol 7. Springer, Singapore

book

33. C. Petchakup, H.M. Tay, K.H.H. Li and H.W. Hou*, Integrated inertial-impedance cytometry for rapid label-free leukocyte isolation and profiling of neutrophil extracellular traps (NETs), Lab on a Chip, 19: p1736-1746, 2019.

2018

32. H.M. Tay, W.H. Yeap, R. Dalan, S.C. Wong* and H.W. Hou*, Multiplex label-free fractionation of peripheral blood mononuclear cells for rapid identification of monocyte-platelet aggregates, Analytical Chemistry, 90(24): 14535-14542, 2018.

31. C. Petchakup, H.M. Tay, W.H. Yeap, R. Dalan, S.C. Wong, K.H.H. Li* and H.W. Hou*, Label-free leukocyte sorting and impedance-based profiling for diabetes testing, Biosensors and Bioelectronics, 118: p.195-203, 2018.

30. H.M. Tay, R. Dalan, K.H.H. Li, B.O. Boehm* and H.W. Hou*, An integrated microdevice  for neutrophil functional phenotyping in diabetes testing, Small, 14(6): 1702832, 2018.

A Novel Microdevice for Rapid Neutrophil Purification and Phenotyping in Type 2 Diabetes Mellitus FRONTISPIECE

29. N.V. Menon, H.M. Tay, K.T. Pang, R. Dalan, S.C. Wong, X.M. Wang, K.H.H. Li and H.W. Hou*, A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis, APL Bioengineering, 2(1): 016103, 2018.

2017

28. C.H. Lim, M. Puthia, M. Butrym, H.M. Tay, M.Z.Y. Lee, H.W. Hou and A. Schmidtchen, Thrombin-derived host defence peptide modulates neutrophil rolling and migration in vitro and functional response in vivo. Scientific Reports, 7, 2017.

27. N.V. Menon, H.M. Tay, S.N. Wee, K.H.H. Li and H.W. Hou*, Micro-engineered perfusable 3D vasculature for cardiovascular diseases. Lab on a Chip, 17(17): p. 2960-2968, 2017.

26. H.M. Tay, S. Kharel, R. Dalan, Z.J. Chen, K.K. Tan, B.O. Boehm, S.C.J. Loo and H.W. Hou*, Rapid purification of sub-micrometer particles for enhanced drug release and microvesicles isolation. NPG Asia Materials, 9: e434, 2017.

25. H.W. Hou, H.M. Tay, R. Dalan and B.O. Boehm, Advances in neutrophil testing in type 2 diabetes mellitus. Current Research in Diabetes & Obesity Journal, 1(5): p.555572, 2017.

24. X.Liu, X. Huang, Y. Jiang, H. Xu, J. Guo, H.W. Hou, M. Yan, H. Yu, A microfluidics cytometer for complete blood count with a 3.2 megapixel, 1.1-um-pitch super resolution image sensor in 65-nm BSI CMOS. IEEE Trans. Biomed. Circuits and Systems, 11(4): 794-803, 2017.

23. C.Petchakup, K.H.H. Li*, and H.W. Hou*, Advances in single cell impedance cytometry for biomedical applications. Micromachines, 8(3): p. 87, 2017.

22. Y.L. Fan, H.W. Hou, H.M. Tay, W.M. Guo, P.O. Berggren, S.C.J. Loo, Preservation of anti-cancer and immunosuppressive properties of rapamycin achieved through controlled releasing particles. AAPS PharmSciTech, 18(7): p. 2648-2657, 2017.

21. S.M. Davidson, O. Jonas, M.A. Keibler, H.W. Hou, A. Luengo, J.R. Mayers, J. Wyckoff, A.D. Rosario, M. Whitman, C.R. Chin, K.J. Condon, A. Lammers, K.A. Kellersberger, B.J. Stall, G. Stephanopoulos, D. Bar-Sagi, J. Han, J.D. Rabinowitz, M. Cima, R. Langer and M.G. Vander Heiden, Direct evidence for cancer cell-autonomous extracellular protein catabolism in pancreatic tumors. Nature Medicine, 23, 235-241, 2017.

2016

20. H.W. Hou, C. Petchakup, H.M. Tay, Z.Y. Tam, R. Dalan, D.E.K. Chew, K.H.H. Li and B.O. Boehm, Rapid and label-free microfluidic neutrophil purification and phenotyping in diabetes mellitus. Scientific Reports, 6, 2016.

19. J.L. Prieto, H.W. Su, H.W. Hou, M.P. Vera, B.D. Levy, R.M. Baron, J. Han and J. Voldman, Monitoring sepsis using electrical profiling. Lab on a Chip, 2016. 16(22): p. 4333-4340, 2016.

18. A.Sarkar, H.W. Hou, E. Mahan, J. Han and G. Alter, Multiplexed affinity-based separation of proteins and cells using inertial microfluidics. Scientific Reports, 6, 2016.

17. H.M. Tay, D. Yeo, C. Wiraja, C.J. Xu and H.W. Hou*, Microfluidic buffer exchange for interference-free micro/nanoparticle cell engineering. Journal of Visualized Experiments, 113: e54327, 2016.

16. H.W. Hou, L. Wu, D.P. Amador-Munoz, M.P. Vera, A. Coronata, J.A. Englert, B.D. Levy, R.M. Baron and J. Han, Broad spectrum immunomodulation using biomimetic blood cell margination for sepsis therapy. Lab on a Chip,16(4): p. 688-699, 2016.

2015

15. D.Yeo, C. Wiraja, Y.Y. Zhou, H.M. Tay, C.J. Xu* and H.W. Hou*, Interference-free micro/nano-particle cell engineering using high-throughput microfluidic separation. ACS Applied Materials & Interfaces, 7(37): p. 20855-20864, 2015.

14. H.W. Hou, R.P. Bhattacharyya, D.T. Hung and J. Han, Direct detection and drug-resistance profiling of bacteremias using inertial microfluidics. Lab on a Chip, 15(10): p. 2297-2307, 2015.

  •  Press articles
    • Repurposed Microfluidic CTC purification Tech Enables Blood-based Antibiotic Resistance Test by Genomeweb

13. S.Huang, H.W. Hou, T. Kanias, J.T. Sertorio, H. Chen, D. Sinchar, M.T. Gladwin and J. Han, Towards microfluidic-based depletion of stiff and fragile human red cells that accumulate during blood storage. Lab on a Chip, 15(2): p. 448-458, 2015.

12. C.M. Birch, H.W. Hou, J. Han, J.C. Niles, I-SELEX: Efficient aptamer selection using inertial microfluidics. Scientific Reports, 5, 2015.

11. T.F. Kong, W. Ye, W.K. Peng, H.W. Hou, Marcos, P.R. Preiser, N.T. Nguyen, J. Han, Enhancing malaria diagnosis through microfluidic cell enrichment and magnetic resonance relaxometry detection.Scientific Reports, 5, 2015.

2009- 2014 (Prior NTU)

10. H.W. Hou, M.E. Warkiani, B.L. Khoo, Z.R. Li, R.A. Soo, D.S.W. Tan, W.T. Lim, J. Han, A.A.S. Bhagat and C.T. Lim, Isolation and retrieval of circulating tumor cells using centrifugal forces. Scientific Reports, 3, 2013.

  • Press articles
    • Spinning Out In Control by Cancerforall
    • Capturing Cancer Cells on the Move by TheScientist
    • Highly Cited Paper- Top 1% in Clinical Medicine (Web of Science)

9. L.Wu, G.F. Guan, H.W. Hou, A.A.S. Bhagat, and J. Han, Separation of leukocytes from blood using spiral channel with trapezoid cross-section. Analytical Chemistry, 84, 9324-9331, 2012.

8. H.W. Hou, H.Y. Gan, A.A.S. Bhagat, L.D. Li, C.T. Lim, and J. Han, A microfluidics approach towards high-throughput pathogen removal from blood using margination. Biomicrofluidics, 6(2):p. 024115-024128, 2012.

7. H.W. Hou, W.C. Lee, M.C. Leong, S. Sonam, S.R.K. Vedula, and C.T. Lim, Microfluidics for applications in cell mechanics and mechanobiology. Cellular and Molecular Bioengineering, 4(4): p. 591-602, 2011.

6. H.W. Hou, A.A.S. Bhagat, W.C. Lee, S. Huang, J. Han and C. T. Lim, Microfluidic devices for blood fractionation, Micromachines, 2(3): p. 319-343, 2011.

5. P.K. Ang, A. Li, M. Jaiswal, Y. Wang, H.W. Hou, J.T.L. Thong, C.T. Lim, and K.P. Loh, Flow Sensing of Single Cell by Graphene Transistor in a Microfluidic Channel. Nano Letters, 11(12):p. 5240–5246, 2011.

4. A.A.S. Bhagat, H.W. Hou, L.D. Li, C.T. Lim, and J. Han, Pinched flow coupled shear-modulated inertial microfluidics for high-throughput rare blood cell separation. Lab on a Chip, 11(11): p. 1870-1878, 2011.

3. A.A.S. Bhagat, H. Bow, H.W. Hou, S.J. Tan, C.T. Lim and J. Han, Microfluidics for cell separation. Medical and Biological Engineering and Computing, 2010. 48(10): p. 999-1014, 2010.

  • Highly Cited Paper- Top 1% in Engineering (Web of Science)

2. H.W. Hou, A.A.S. Bhagat, A.G.L. Chong, P. Mao, K.S.W. Tan, J. Han and C. T. Lim, Deformability based cell margination – A simple microfluidic design for malaria infected erythrocyte separation. Lab on a Chip, 10(19): p. 2605-2613, 2010.

1. H.W. Hou, Q.S. Li, G.Y.H. Lee, A.P. Kumar, C.N. Ong, and C.T. Lim, Deformability study of breast cancer cells using microfluidics. Biomedical Microdevices, 11(3): 557-564, 2009.