Publications

2023

52. N. Lu, H.M. Tay, C. Petchakup, L. He, L. Gong, K.K. Maw, S.Y. Leong, W.W Lok, H.B. Ong, R. Guo, K.H.H. Li, H.W. Hou, Label-free Microfluidic Cell Sorting and Detection for Rapid Blood Analysis, Lab on a Chip, Advance Article, 23, 1226 – 1257, 2023.

51. Y. Deng, H.M. Tay, Y. Zhou, X. Fei, X. Tang, M. Nishikawa, Y. Yatomi, H.W. Hou, T-H. Xiao, K. Goda, Studying the efficacy of antiplatelet drugs on atherosclerosis by optofluidic imaging on a chip, Lab on a Chip, Advance Article, 23, 410-420, 2023.

2022

50. C. Petchakup, H. Yang, L. Gong, L. He, H.M. Tay, R. Dalan, A.J. Chung, K.H.H. Li, H.W. Hou, Microfluidic Impedance-Deformability Cytometry for Label-Free Single Neutrophil Mechanophenotyping, Small, 18, 2104822, 2022.

  • Press articles
    • Featured as Small Back Cover!

49. K.X. Wu, N.J.Y Yeo, C.Y. Ng, F.W.J. Chioh, F. Qiao, X. Tian, B. Yang, G. Narayanan, H.M. Tay, H.W. Hou, N.R. Dunn, X. Su, C.G. Cheung, C. Cheung, Hyaluronidase-1-mediated glycocalyx impairment underlies endothelial abnormalities in polypoidal choroidal vasculopathy, BMC Biology, 20, 47, 2022.

48. Y. Deng, J.A. Duque, C. Su, Y. Zhou, M. Nishikawa, T. Xiao, Y. Yatomi, H.W. Hou and K. Goda, Understanding stenosis-induced platelet aggregation on a chip by high-speed optical imaging, Sensors and Actuators B: Chemical, 356: 131318, 2022.

47. S.Y. Leong, H.B. Ong, H.M. Tay, F. Kong, M. Upadya, L. Gong, M. Dao and H.W.Hou, Microfluidic Size Exclusion Chromatography (μSEC) for Extracellular Vesicles and Plasma Protein Separation, Small, 2104470, 2022.

2021

46. C. Su, Y.J. Chuah, H.B. Ong, H.M. Tay R. Dalan and H.W. Hou, A Facile and Scalable Hydrogel Patterning Method for Microfluidic 3D Cell Culture and Spheroid-in-Gel Culture Array, Biosensors, 11(12): 509, 2021.

45. L. Zhang, T. Xu, J. Zhang, S. Wong, M. Ritchie, H.W. Hou and Y. Wang, Single Cell Metabolite Detection Using Inertial Microfluidics Assisted Ion Mobility Mass Spectroscopy, Analytical Chemistry, 93, 30, 10462 – 10468, 2021.

44. K.J. Puan, B.S. Luis, N. Yusof, D. Kumar, A.K. Andiappan, W. Lee, S. Cajic, D. Vuckovic, J.D. Chan, T. Döllner, H.W. Hou, Y. Jiang, C. Tian, the 23andMe Research Team, E. Rapp, M. Poidinger, D.Y. Wang, N. Soranzo, B. Lee and O. Rötzschke, FUT6 deficiency compromises basophil function by selectively abrogating their sialyl-Lewis x expression, Communications Biology, 4:832, 2021.

43. K.T. Pang, M. Ghim, C. Liu, H.M. Tay, C.W. Fhu, R.N. Chia, B. Qiu, P. Sarathchandra, A. Chester, M. Yacoub, F.L. Wilkinson, R. Weston, C.M. Warboys, H.W. Hou, P.D. Weinberg and X. Wang, Leucine-rich α-2-glycoprotein 1 suppresses endothelial cell activation through ADAM10-mediated shedding of TNF-α receptor, Frontiers in Cell and Developmental Biology, 9:706143, 2021.

42. H.M. Tay, S.Y. Leong, X. Xu, F. Kong, M. Upadya, R. Dalan, C.Y. Tay, M. Dao, S. Suresh, H.W Hou, Direct Isolation of Circulating Extracellular Vesicles from Blood for Vascular Risk Profiling in Type 2 Diabetes Mellitus, Lab on a Chip, 21: 2511, 2021.

41. C. Su, N.V. Menon, X. Xu, Y.R. Teo, H. Cao, R. Dalan, C.Y. Tay, H.W Hou, A novel human arterial wall-on-a-chip to study endothelial inflammation and vascular smooth muscle cell migration in early atherosclerosis, Lab on a Chip, 21: 2359-2371, 2021.

  • Press articles
    • Featured as Lab on a Chip back cover
    • Singapore builds 3D model to study cardiovascular diseases – by Biospectrum Asia
    • 3D model to study vascular diseases – by Mirage News
    • Singapore researchers develop novel 3D model to study vascular diseases – by Vascular News
    • Novel 3D Model Aids Vascular Disease Research – by Labmate Online
    • 新加坡研究人员开发了新的3D模型来研究血管疾病 – by e-Biotrade
    • 3D model to study vascular diseases – by NTU

40. C. Petchakup, P.E. Hutchinson, H.M. Tay, S.Y. Leong, K.H.H. Li, H.W. Hou, Label-free quantitative lymphocyte activation profiling using microfluidic impedance cytometry, Sensors and Actuators B: Chemical, 339: 129864, 2021.

39. L. Gong, C. Petchakup, P. Shi, P.L. Tan, L.P. Tan, C.Y. Tay, H.W. Hou, Direct and Label‐Free Cell Status Monitoring of Spheroids and Microcarriers Using Microfluidic Impedance Cytometry, Small, 2007500, 2021.

  • Press articles
    • NTU researchers developed a non-invasive and label-free microfluidic impedance biosensor for cellular monitoring in biomanufacturing – by NTU MAE News
    • Featured as Small Frontispiece

2020

38. C. Liu, M.H.Y. Teo, S.L.T. Pek, Xi. Wu, M.L. Leong, H.M. Tay, H.W. Hou, C. Ruedl, S.E. Moss, J. Greenwood, S. Tavintharan, W. Hong, X. Wang, A Multifunctional Role of Leucine-Rich-Alpha 2 Glycoprotein 1 in Cutaneous Wound Healing Under Normal and Diabetic Conditions, Diabetes, 69(11): 2467-2480, 2020.

37. Z. Wu, H.K. Lim, S.J. Tan, A. Gautam, H.W. Hou, K.W. Ng, N.S. Tan, C.Y. Tay, Potent‐By‐Design: Amino Acids Mimicking Porous Nanotherapeutics with Intrinsic Anticancer Targeting Properties, Small, 16(34): 2003757, 2020.

36. N. Menon, C. Su, K.T. Pang, Z.J. Phua, H.M. Tay, R. Dalan, X. Wang, K.H.H. Li and H.W. Hou, Recapitulating Atherogenic Flow Disturbances and Vascular Inflammation in a Perfusable 3D Stenosis Model, Biofabrication, 12(4): 045009, 2020.

35. H.M. Tay, W.H. Yeap, R. Dalan, S.C. Wong and H.W. Hou, Increased monocyte‐platelet aggregates and monocyte‐endothelial adhesion in healthy individuals with vitamin D deficiency, FASEB, 34(8): 11133-11142, 2020.

2019

34. N. Liu, C. Petchakup., H.M. Tay, K.H.H. Li, H.W. Hou (2019) Spiral Inertial Microfluidics for Cell Separation and Biomedical Applications. In: Tokeshi M. (eds) Applications of Microfluidic Systems in Biology and Medicine, Bioanalysis (Advanced Materials, Methods, and Devices), vol 7. Springer, Singapore

book

33. C. Petchakup, H.M. Tay, K.H.H. Li and H.W. Hou, Integrated inertial-impedance cytometry for rapid label-free leukocyte isolation and profiling of neutrophil extracellular traps (NETs), Lab on a Chip, 19: 1736-1746, 2019.

2018

32. H.M. Tay, W.H. Yeap, R. Dalan, S.C. Wong and H.W. Hou, Multiplex label-free fractionation of peripheral blood mononuclear cells for rapid identification of monocyte-platelet aggregates, Analytical Chemistry, 90(24): 14535-14542, 2018.

31. C. Petchakup, H.M. Tay, W.H. Yeap, R. Dalan, S.C. Wong, K.H.H. Li and H.W. Hou, Label-free leukocyte sorting and impedance-based profiling for diabetes testing, Biosensors and Bioelectronics, 118: 195-203, 2018.

30. H.M. Tay, R. Dalan, K.H.H. Li, B.O. Boehm and H.W. Hou, A novel microdevice for rapid neutrophil purification and phenotyping in type 2 diabetes mellitus, Small, 14(6): 1702832, 2018.

29. N.V. Menon, H.M. Tay, K.T. Pang, R. Dalan, S.C. Wong, X.M. Wang, K.H.H. Li and H.W. Hou, A tunable microfluidic 3D stenosis model to study leukocyte-endothelial interactions in atherosclerosis, APL Bioengineering, 2(1): 016103, 2018.

2014- 2017 (LKCMedicine @ NTU)

28. C.H. Lim, M. Puthia, M. Butrym, H.M. Tay, M.Z.Y. Lee, H.W. Hou and A. Schmidtchen, Thrombin-derived host defence peptide modulates neutrophil rolling and migration in vitro and functional response in vivo, Scientific Reports, 7: 11201, 2017.

27. N.V. Menon, H.M. Tay, S.N. Wee, K.H.H. Li and H.W. Hou, Micro-engineered perfusable 3D vasculature for cardiovascular diseases, Lab on a Chip, 17(17): 2960-2968, 2017.

26. H.M. Tay, S. Kharel, R. Dalan, Z.J. Chen, K.K. Tan, B.O. Boehm, S.C.J. Loo and H.W. Hou, Rapid purification of sub-micrometer particles for enhanced drug release and microvesicles isolation, NPG Asia Materials, 9: e434, 2017.

25. H.W. Hou, H.M. Tay, R. Dalan and B.O. Boehm, Advances in neutrophil testing in type 2 diabetes mellitus, Current Research in Diabetes & Obesity Journal, 1(5): 555572, 2017.

24. X.Liu, X. Huang, Y. Jiang, H. Xu, J. Guo, H.W. Hou, M. Yan, H. Yu, A microfluidics cytometer for complete blood count with a 3.2 megapixel, 1.1-um-pitch super resolution image sensor in 65-nm BSI CMOS, IEEE Trans. Biomed. Circuits and Systems, 11(4): 794-803, 2017.

23. C.Petchakup, K.H.H. Li, and H.W. Hou, Advances in single cell impedance cytometry for biomedical applications, Micromachines, 8(3): 87, 2017.

22. Y.L. Fan, H.W. Hou, H.M. Tay, W.M. Guo, P.O. Berggren, S.C.J. Loo, Preservation of anti-cancer and immunosuppressive properties of rapamycin achieved through controlled releasing particles, AAPS PharmSciTech, 18(7): 2648-2657, 2017.

21. S.M. Davidson, O. Jonas, M.A. Keibler, H.W. Hou, A. Luengo, J.R. Mayers, J. Wyckoff, A.D. Rosario, M. Whitman, C.R. Chin, K.J. Condon, A. Lammers, K.A. Kellersberger, B.J. Stall, G. Stephanopoulos, D. Bar-Sagi, J. Han, J.D. Rabinowitz, M. Cima, R. Langer and M.G. Vander Heiden, Direct evidence for cancer cell-autonomous extracellular protein catabolism in pancreatic tumors, Nature Medicine, 23: 235-241, 2017.

2016

20. H.W. Hou, C. Petchakup, H.M. Tay, Z.Y. Tam, R. Dalan, D.E.K. Chew, K.H.H. Li and B.O. Boehm, Rapid and label-free microfluidic neutrophil purification and phenotyping in diabetes mellitus, Scientific Reports, 6: 29410, 2016.

19. J.L. Prieto, H.W. Su, H.W. Hou, M.P. Vera, B.D. Levy, R.M. Baron, J. Han and J. Voldman, Monitoring sepsis using electrical profiling, Lab on a Chip, 2016. 16(22): 4333-4340, 2016.

18. A.Sarkar, H.W. Hou, E. Mahan, J. Han and G. Alter, Multiplexed affinity-based separation of proteins and cells using inertial microfluidics, Scientific Reports, 6: 23589, 2016.

17. H.M. Tay, D. Yeo, C. Wiraja, C.J. Xu and H.W. Hou, Microfluidic buffer exchange for interference-free micro/nanoparticle cell engineering, Journal of Visualized Experiments, 113: e54327, 2016.

16. H.W. Hou, L. Wu, D.P. Amador-Munoz, M.P. Vera, A. Coronata, J.A. Englert, B.D. Levy, R.M. Baron and J. Han, Broad spectrum immunomodulation using biomimetic blood cell margination for sepsis therapy, Lab on a Chip,16(4): 688-699, 2016.

2015

15. D.Yeo, C. Wiraja, Y.Y. Zhou, H.M. Tay, C.J. Xu and H.W. Hou, Interference-free micro/nano-particle cell engineering using high-throughput microfluidic separation, ACS Applied Materials & Interfaces, 7(37): 20855-20864, 2015.

14. H.W. Hou, R.P. Bhattacharyya, D.T. Hung and J. Han, Direct detection and drug-resistance profiling of bacteremias using inertial microfluidics, Lab on a Chip, 15(10): 2297-2307, 2015.

  •  Press articles
    • Repurposed Microfluidic CTC purification Tech Enables Blood-based Antibiotic Resistance Test by Genomeweb

13. S.Huang, H.W. Hou, T. Kanias, J.T. Sertorio, H. Chen, D. Sinchar, M.T. Gladwin and J. Han, Towards microfluidic-based depletion of stiff and fragile human red cells that accumulate during blood storage, Lab on a Chip, 15(2): 448-458, 2015.

12. C.M. Birch, H.W. Hou, J. Han, J.C. Niles, Identification of malaria parasite-infected red blood cell surface aptamers by inertial microfluidic SELEX (I-SELEX), Scientific Reports, 5: 11347, 2015.

11. T.F. Kong, W. Ye, W.K. Peng, H.W. Hou, Marcos, P.R. Preiser, N.T. Nguyen, J. Han, Enhancing malaria diagnosis through microfluidic cell enrichment and magnetic resonance relaxometry detection, Scientific Reports, 5: 11425, 2015.

2009- 2014 (Prior NTU)

10. H.W. Hou, M.E. Warkiani, B.L. Khoo, Z.R. Li, R.A. Soo, D.S.W. Tan, W.T. Lim, J. Han, A.A.S. Bhagat and C.T. Lim, Isolation and retrieval of circulating tumor cells using centrifugal forces, Scientific Reports, 3: 1259, 2013.

  • Press articles
    • Spinning Out In Control by Cancerforall
    • Capturing Cancer Cells on the Move by TheScientist
    • Highly Cited Paper- Top 1% in Clinical Medicine (Web of Science)

9. L.Wu, G.F. Guan, H.W. Hou, A.A.S. Bhagat, and J. Han, Separation of leukocytes from blood using spiral channel with trapezoid cross-section, Analytical Chemistry, 84: 9324-9331, 2012.

8. H.W. Hou, H.Y. Gan, A.A.S. Bhagat, L.D. Li, C.T. Lim, and J. Han, A microfluidics approach towards high-throughput pathogen removal from blood using margination, Biomicrofluidics, 6(2): 024115-024128, 2012.

7. H.W. Hou, W.C. Lee, M.C. Leong, S. Sonam, S.R.K. Vedula, and C.T. Lim, Microfluidics for applications in cell mechanics and mechanobiology, Cellular and Molecular Bioengineering, 4(4): 591-602, 2011.

6. H.W. Hou, A.A.S. Bhagat, W.C. Lee, S. Huang, J. Han and C. T. Lim, Microfluidic devices for blood fractionation, Micromachines, 2(3): 319-343, 2011.

5. P.K. Ang, A. Li, M. Jaiswal, Y. Wang, H.W. Hou, J.T.L. Thong, C.T. Lim, and K.P. Loh, Flow Sensing of Single Cell by Graphene Transistor in a Microfluidic Channel, Nano Letters, 11(12): 5240–5246, 2011.

4. A.A.S. Bhagat, H.W. Hou, L.D. Li, C.T. Lim, and J. Han, Pinched flow coupled shear-modulated inertial microfluidics for high-throughput rare blood cell separation, Lab on a Chip, 11(11): 1870-1878, 2011.

3. A.A.S. Bhagat, H. Bow, H.W. Hou, S.J. Tan, C.T. Lim and J. Han, Microfluidics for cell separation, Medical and Biological Engineering and Computing, 48(10): 999-1014, 2010.

  • Highly Cited Paper- Top 1% in Engineering (Web of Science)

2. H.W. Hou, A.A.S. Bhagat, A.G.L. Chong, P. Mao, K.S.W. Tan, J. Han and C. T. Lim, Deformability based cell margination – A simple microfluidic design for malaria infected erythrocyte separation, Lab on a Chip, 10(19): 2605-2613, 2010.

1. H.W. Hou, Q.S. Li, G.Y.H. Lee, A.P. Kumar, C.N. Ong, and C.T. Lim, Deformability study of breast cancer cells using microfluidics. Biomedical Microdevices, 11(3): 557-564, 2009.